development of ph sensitive nanoparticles for intestinal drug delivery using chemically modified guar gum co-polymer

the aim of the research work was to chemically modify guar gum(gg) as a ph sensitive co-polymer and formulating intestinal targeting eso nanoparticles (nps) using the synthesized co-polymer. poly acrylamide-grafted-guar gum (paam-g-gg) co-polymer was synthesized by free radical polymerization. chemical modification of paam-g-gg by alkaline hydrolysis results in formation of a ph-sensitive co-polymer. the effect of gg and acryl amide (aam) on grafting was studied. esomeprazole magnesium (eso) loaded ph sensitive nps were prepared by nano-emulsification polymer crosslinking method and characterized. sixteen formulations were prepared and the concentration of process variables was varied to obtain nanoparticles of 200-600nm. the nps were found to be homogenous in size distribution. the encapsulation efficiency and drug loading ranged from 33.2% to 50.1% and 12.2% to 17.2% respectively. particle size, encapsulation efficiency and drug loading increased along with co-polymer concentration. in-vitro release studies at ph 1.2 for 2 h, followed by ph 6.8 showed that environment ph significantly affected the drug release. sem has shown that nps are spherical with smooth surface. the ph sensitive paam-g-ggnps resisted the initial release of the drug from the drug loaded nps in acidic ph and delayed the release process to a longer period in alkaline environment.